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pathscan multiplex western cocktail i kit  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc pathscan multiplex western cocktail i kit
    Pathscan Multiplex Western Cocktail I Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pathscan+multiplex+western+cocktail+i+kit/pathscan++multiplex+western+cocktail+i+detection+kit/us08535897-878-69-75
    Average 90 stars, based on 1 article reviews
    pathscan multiplex western cocktail i kit - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Multiplex Assay:

    Article Title: Resveratrol downregulates PI3K/Akt/mTOR signaling pathways in human U251 glioma cells
    Article Snippet: PI3K inhibitor LY294002 (2-(4-Morpholinyl)-8-phenyl-4H-1-benzopyran-4-one), Akt inhibitor (SH-6, phosphoinositide analogue inhibitor), and mTOR inhibitor rapamycin were obtained from Calbiochem (La Jolla, CA). .. Antibodies against phospho-mTOR (ser2448), phospho-Akt (ser473), Akt, cleaved caspase-3 (Asp-175) and PathScan multiplex Western cocktail I kit (phospho-p90RSK, phospho-Akt, phospho-p44/42 MAPK and phospho-S6 ribosomal protein) were obtained from Cell Signaling Technology (Beverly, MA). .. Antibodies against cyclin D1 and actin (I-19) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA) and antibody against poly(ADP-ribose) polymerase (PARP) was purchased from Oncogene Research Products (Cambridge, MA).

    Article Title: Assays for non-apoptotic cell death and uses thereof
    Article Snippet: .. Samples were separated using SDS-PAGE, transferred to a PVDF membrane, blocked for 1 hour at room temperature in Licor Odyssey Blocking Buffer and incubated with the necessary primary and secondary antibodies: anti-VDAC1 (Abcam, #ab3434), anti-VDAC1 (Calbiochem, #529534), anti-VDAC2 (Abcam, #ab22170), anti-eIF4E (Santa Cruz Biotechnology, #sc-9976), anti-α-Tubulin (Sigma, #T6199), anti-actin (Santa Cruz Biotechnology, #1616R), IRDye 800 goat anti-rabbit antibody (Rockland Immunochemicals, #611-132-122), Alexa Fluor 680 goat anti-mouse (Molecular Probes, #A21058), PathScan Multiplex Western Cocktail I Kit (Cell Signaling Technology), anti-PARP (Abeam, #ab105). .. Membranes were scanned using the Licor OdysseyTM Imaging System.

    Article Title: Prostaglandin E2 Increases Cardiac Fibroblast Proliferation and Increases Cyclin D Expression via EP1 Receptor
    Article Snippet: .. To determine the influence of PGE 2 on various signaling pathways, we used the PathScan Multiplex Western Cocktail I kit (Cell Signaling). ..

    Western Blot:

    Article Title: Resveratrol downregulates PI3K/Akt/mTOR signaling pathways in human U251 glioma cells
    Article Snippet: PI3K inhibitor LY294002 (2-(4-Morpholinyl)-8-phenyl-4H-1-benzopyran-4-one), Akt inhibitor (SH-6, phosphoinositide analogue inhibitor), and mTOR inhibitor rapamycin were obtained from Calbiochem (La Jolla, CA). .. Antibodies against phospho-mTOR (ser2448), phospho-Akt (ser473), Akt, cleaved caspase-3 (Asp-175) and PathScan multiplex Western cocktail I kit (phospho-p90RSK, phospho-Akt, phospho-p44/42 MAPK and phospho-S6 ribosomal protein) were obtained from Cell Signaling Technology (Beverly, MA). .. Antibodies against cyclin D1 and actin (I-19) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA) and antibody against poly(ADP-ribose) polymerase (PARP) was purchased from Oncogene Research Products (Cambridge, MA).

    Article Title: Assays for non-apoptotic cell death and uses thereof
    Article Snippet: .. Samples were separated using SDS-PAGE, transferred to a PVDF membrane, blocked for 1 hour at room temperature in Licor Odyssey Blocking Buffer and incubated with the necessary primary and secondary antibodies: anti-VDAC1 (Abcam, #ab3434), anti-VDAC1 (Calbiochem, #529534), anti-VDAC2 (Abcam, #ab22170), anti-eIF4E (Santa Cruz Biotechnology, #sc-9976), anti-α-Tubulin (Sigma, #T6199), anti-actin (Santa Cruz Biotechnology, #1616R), IRDye 800 goat anti-rabbit antibody (Rockland Immunochemicals, #611-132-122), Alexa Fluor 680 goat anti-mouse (Molecular Probes, #A21058), PathScan Multiplex Western Cocktail I Kit (Cell Signaling Technology), anti-PARP (Abeam, #ab105). .. Membranes were scanned using the Licor OdysseyTM Imaging System.

    Article Title: Prostaglandin E2 Increases Cardiac Fibroblast Proliferation and Increases Cyclin D Expression via EP1 Receptor
    Article Snippet: .. To determine the influence of PGE 2 on various signaling pathways, we used the PathScan Multiplex Western Cocktail I kit (Cell Signaling). ..

    Membrane:

    Article Title: Assays for non-apoptotic cell death and uses thereof
    Article Snippet: .. Samples were separated using SDS-PAGE, transferred to a PVDF membrane, blocked for 1 hour at room temperature in Licor Odyssey Blocking Buffer and incubated with the necessary primary and secondary antibodies: anti-VDAC1 (Abcam, #ab3434), anti-VDAC1 (Calbiochem, #529534), anti-VDAC2 (Abcam, #ab22170), anti-eIF4E (Santa Cruz Biotechnology, #sc-9976), anti-α-Tubulin (Sigma, #T6199), anti-actin (Santa Cruz Biotechnology, #1616R), IRDye 800 goat anti-rabbit antibody (Rockland Immunochemicals, #611-132-122), Alexa Fluor 680 goat anti-mouse (Molecular Probes, #A21058), PathScan Multiplex Western Cocktail I Kit (Cell Signaling Technology), anti-PARP (Abeam, #ab105). .. Membranes were scanned using the Licor OdysseyTM Imaging System.

    Blocking Assay:

    Article Title: Assays for non-apoptotic cell death and uses thereof
    Article Snippet: .. Samples were separated using SDS-PAGE, transferred to a PVDF membrane, blocked for 1 hour at room temperature in Licor Odyssey Blocking Buffer and incubated with the necessary primary and secondary antibodies: anti-VDAC1 (Abcam, #ab3434), anti-VDAC1 (Calbiochem, #529534), anti-VDAC2 (Abcam, #ab22170), anti-eIF4E (Santa Cruz Biotechnology, #sc-9976), anti-α-Tubulin (Sigma, #T6199), anti-actin (Santa Cruz Biotechnology, #1616R), IRDye 800 goat anti-rabbit antibody (Rockland Immunochemicals, #611-132-122), Alexa Fluor 680 goat anti-mouse (Molecular Probes, #A21058), PathScan Multiplex Western Cocktail I Kit (Cell Signaling Technology), anti-PARP (Abeam, #ab105). .. Membranes were scanned using the Licor OdysseyTM Imaging System.

    Incubation:

    Article Title: Assays for non-apoptotic cell death and uses thereof
    Article Snippet: .. Samples were separated using SDS-PAGE, transferred to a PVDF membrane, blocked for 1 hour at room temperature in Licor Odyssey Blocking Buffer and incubated with the necessary primary and secondary antibodies: anti-VDAC1 (Abcam, #ab3434), anti-VDAC1 (Calbiochem, #529534), anti-VDAC2 (Abcam, #ab22170), anti-eIF4E (Santa Cruz Biotechnology, #sc-9976), anti-α-Tubulin (Sigma, #T6199), anti-actin (Santa Cruz Biotechnology, #1616R), IRDye 800 goat anti-rabbit antibody (Rockland Immunochemicals, #611-132-122), Alexa Fluor 680 goat anti-mouse (Molecular Probes, #A21058), PathScan Multiplex Western Cocktail I Kit (Cell Signaling Technology), anti-PARP (Abeam, #ab105). .. Membranes were scanned using the Licor OdysseyTM Imaging System.

    Protein-Protein interactions:

    Article Title: Prostaglandin E2 Increases Cardiac Fibroblast Proliferation and Increases Cyclin D Expression via EP1 Receptor
    Article Snippet: .. To determine the influence of PGE 2 on various signaling pathways, we used the PathScan Multiplex Western Cocktail I kit (Cell Signaling). ..



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